dATP, or 2'-deoxyadenosine 5'-triphosphate, is an essential compound for many different types of experiments within molecular biology. This nucleotide acts as a building block in the synthesis of DNA, especially during processes like PCR (polymerase chain reaction), DNA sequencing and various other cloning methods. A 100mM solution of dATP refers to a concentrated form of this molecule typically made up in high purity water so that it can be used for a wide range of biological reactions.
The 100mM dATP solution finds use in numerous molecular biology protocols. It is absolutely necessary for PCR, where it gets incorporated into the growing strand by DNA polymerase enzymes. It is also heavily utilized in real-time PCR, RT-PCR, cDNA synthesis, primer extension reactions and DNA labeling to name just a few applications. Its great purity level (>99% by HPLC) ensures low levels of impurity which would otherwise inhibit such delicate reactions. In addition to this property however; it also happens that the said product works well with many different kinds of DNA polymerases therefore making life easier on researchers who might otherwise need multiple kinds.
Manufacturers make sure they follow strict quality control measures when producing dATPs so as not only guarantee but also maintain reliability and consistency between batches produced over time within their own company’s framework – Invitrogen’s being no exception since its products are manufactured under ISO 9001 standards inside class D clean rooms. Such conditions help keep track with what should be considered best practices throughout an industry sector while still ensuring safety remains paramount; considering that pH values ranging from around 7.3 through to about 7.5 have been found optimal for most molecular biology applications where these solutions are commonly used another reason why sodium hydroxide may often be employed during titration stages would relate back towards overall performance based upon convenience factors etcetera. Finally, absence nuclease activities coupled with no detectable contaminating DNA from either human or E. coli sources provides an assurance about purity levels achieved during production stages involving dATPs
Tinzyme Co., Limited was founded in 2015, is a professional molecular bio reagent Manufacturer in China. The key products are nucleic acid substances and the enzymes for molecular biology. We have many patents for the nucleic acid and the enzymes. And the monomers of the nucleic are all enzymatically synthesized using our proprietary unique technology. These dNTPs and NTPs are functionally approved for all PCR and related reactions including Real Time PCR, in vitro transcription assays and DNA sequencing in diagnostics and molecular biology. Also the enzymes such as Taq DNA polymerase, M-MLV Reverse Transcriptase, pfu DNA polymerase are all worked very well. The performance of our products is comparable to the top bio companies of the world.
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Datp 100mm solution is a reagent used in various biochemical and molecular biology applications. It typically contains deoxyadenosine triphosphate (dATP) at a concentration of 100 mM, which is a nucleotide essential for DNA synthesis and repair processes.
Datp 100mm solution is commonly used in research settings for DNA polymerase reactions, PCR amplification, and other DNA-related experiments where dATP is required as a substrate for enzymatic activity.
Datp 100mm solution should be stored at temperatures ranging from -20°C to -80°C to maintain its stability and prevent degradation over time.
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