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What is Taq DNA Polymerase?

What is Taq DNA Polymerase?

Taq DNA Polymerase is an enzyme that can withstand high temperatures. It plays a key role in many molecular biology techniques, especially the Polymerase Chain Reaction (PCR). Scientists got this enzyme from Thermus aquaticus, a thermophilic bacterium. Taq DNA Polymerase is known for being able to tolerate extreme heat which is necessary during PCR. Its molecular weight is about 65kD and it has both 5'→3' polymerase activity as well as 5' structure-specific nuclease activity thus making it applicable to different molecular systems.

In PCR, Taq DNA Polymerase synthesizes new DNA strands with existing ones serving as templates. Due to its heat stability, this enzyme can still function even after denaturation at around 94°C which occurs during most cycles of amplification reaction. This unique feature eliminates the need for addition of fresh enzyme after each cycle thereby simplifying and enhancing efficiency of PCR process. The ability of Taq DNA polymerase to tolerate high temperature is attributed to the fact that it comes from organisms like T.aquaticus living in hot springs where there are very high temperatures.

The discovery and isolation of Taq DNA Polymerase marked a turning point in molecular biology because it allowed for easy amplification of genetic material through PCR.This tool has found applications across various disciplines such as genetic engineering, forensics or medical diagnostics since then.Its specificity when amplifying particular sequences of deoxyribonucleic acid has enabled researchers examine genomes at unprecedented levels leading to significant advances in medical science.Taq DNA polymerases remains one of those essential reagents used routinely by scientists worldwide due to their strong nature coupled with ability work under different experimental conditions in molecular biology labs today.

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Tinzyme Co., Limited was founded in 2015,  is a professional molecular bio reagent Manufacturer in China. The key products are nucleic acid substances and the enzymes for molecular biology. We have many patents for the nucleic acid and the enzymes. And the monomers of the nucleic are all enzymatically synthesized using our proprietary unique technology. These dNTPs and NTPs are functionally approved for all PCR and related reactions including Real Time PCR, in vitro transcription assays and DNA sequencing in diagnostics and molecular biology. Also the enzymes such as Taq DNA polymerase, M-MLV Reverse Transcriptase, pfu DNA polymerase are all worked very well. The performance of our products is comparable to the top bio companies of the world.

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At TINZYME, our Poly A RNA carriers excel in unmatched efficiency, ensuring swift and effective delivery of RNA molecules to their target destinations within cells. This efficiency is pivotal for maximizing therapeutic outcomes and minimizing potential side effects.

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The superior stability of TINZYME Poly A RNA carriers is a cornerstone of their effectiveness. Our carriers protect RNA from degradation, extending its therapeutic window and enhancing the overall potency of RNA-based treatments.

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TINZYME Poly A RNA carriers boast versatile compatibility, making them suitable for a wide array of RNA types and therapeutic applications. This adaptability empowers researchers and clinicians with a flexible platform for innovative RNA-based therapies.

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What users say about Tinzyme

I am thrilled with the exceptional quality of the ATP 100mM Solution provided by TINZYME. It's not only highly specialized but also incredibly reliable, making it an invaluable asset to my research.

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Scarlett

The MTaq DNA Polymerase from TINZYME has been a game-changer for my lab. Its high-performance capabilities have significantly improved our direct amplification processes, saving us both time and resources.

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Jacob

I am consistently impressed by the reliability of TINZYME's dUTP 100mM solution. It's a staple in our lab for its purity and consistency, ensuring our experiments run smoothly every time.

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Logan

The competitive pricing offered by TINZYME allows us to invest in high-quality reagents without breaking the bank. Their cost-effective solutions have been a real boon to our research budget.

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Harper

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What is Taq DNA Polymerase?

Taq DNA Polymerase is an enzyme isolated from the thermophilic bacterium Thermus aquaticus, which is commonly used in PCR (Polymerase Chain Reaction) to amplify specific DNA sequences. It has the ability to withstand high temperatures and is essential for DNA replication during PCR.

How does Taq DNA Polymerase work?

Taq DNA Polymerase works by catalyzing the synthesis of new DNA strands from a template DNA sequence. During PCR, it adds deoxyribonucleotides (dNTPs) to the growing DNA strand, extending it in a process known as elongation.

What are the advantages of using Taq DNA Polymerase?

The main advantage of Taq DNA Polymerase is its thermostability, meaning it remains active at high temperatures without the need for thermal denaturation. This allows for efficient and continuous amplification during PCR.

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