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dUTP 100mM solution-Reliable 2′-Deoxythymidine-5′-triphosphate, sodium salt solution
- Introduction
Introduction
dUTP 100mM solution Ultra pure HPLC>99%
Description
Introducing the dUTP 100mM Solution, a premium quality and highly pure 2′-Deoxyuridine-5′-triphosphate (dUTP), sodium salt solution. This ready-to-use reagent is prepared to a concentration of 100mM for seamless integration into molecular biology applications requiring modified nucleotides.
Applications:
Hot Start PCR: In hot start PCR techniques, dUTP replaces dTTP to minimize non-specific amplification, especially when combined with UNG treatment for post-PCR cleanup.
Uracil-Nicking Enzyme (UNE)-Mediated PCR: Used in UNE-based methods that cleave uracil-containing DNA strands to increase specificity or generate nicked circular templates.
Next-Generation Sequencing (NGS) Library Preparation: For preparing NGS libraries where dUTP incorporation enables efficient removal of contaminating adapter dimers or unwanted sequences.
Real-Time PCR (qPCR): To reduce carryover contamination in qPCR experiments by incorporating dUTP into amplified products, which can be degraded by UNG before subsequent runs.
DNA Labeling: dUTP can be biotinylated or fluorescently labeled for detection purposes in blotting techniques or microarray hybridization.
Mutation Detection: Incorporation of dUTP during PCR helps in mismatch analysis due to differential recognition by endonucleases specific to uracil.
Product Name: 2′-Deoxyuridine-5′-triphosphate, sodium salt solution
Molecular Formula:C9H12N2Na3O14P3
Molecular Weight: 534.1
CAS Number: 102814-08-4
| TEST | SPECIFICATION |
| Appearance | Clear colorless solution |
| Concentration | 100mM±3% |
| Purity (HPLC area%) | ≥99% |
| λmax | 262nm±2nm |
| Absorbance Rations | A250/A260 0.72~0.78 |
| (pH 7.0) | A280/A260 0.34~0.40 |
| pH (22~25°C) | 7.0±0.1 |
Quality control
1.The concentration is verified by optical density spectrophotometry.
2.This preparation is free of DNase and RNase contamination.
3.The purity of this preparation is determined by HPLC. The minimum passing specification for the triphosphate content is 99%.
4.This lot of dUTP has been performance tested with Taq DNA polymerase and Pfu DNA polymerase.
5.This lot of dUTP has performance tested with Q-PCR.
Storage Recommendations
Long term (Infrequent use; 1-2 times per month): -70°C
Daily/Weekly use: -20°C
Always avoid freeze-thaw cycles or exposure to frequent temperature changes.
These fluctuations can greatly alter product stability.
Standard package size
1ml/vial
10ml/vial
25ml/bottle
50ml/bottle
Other package sizes are available
Delivery Time
2 working days
