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dGTP 100mM solution-Reliable 2′-Deoxyadenosine-5′-triphosphate, sodium salt solution
- Introduction
Introduction
dGTP 100mM solution Ultra pure HPLC>99%
Description
Introducing the dGTP 100mM Solution, a premium quality and ultra-high purity 2′-Deoxyguanosine-5′-triphosphate (dGTP), sodium salt formulation. This ready-to-use reagent is expertly prepared at a concentration of 100mM to deliver unparalleled reliability in molecular biology applications that necessitate authentic nucleotide substrates.
Applications:
Polymerase Chain Reaction (PCR): An essential component for the accurate incorporation of guanine bases during the amplification of DNA sequences in PCR reactions.
DNA Sequencing: One of the four deoxynucleotide triphosphates required for Sanger sequencing and next-generation sequencing methods, where it provides the guanine base for chain elongation.
Real-Time Quantitative PCR (qPCR): Vital for quantifying specific DNA targets through template-dependent amplification by providing the necessary dGTP.
Gene Synthesis and Cloning: A critical substrate for in vitro gene assembly, site-directed mutagenesis, and cloning procedures involving DNA polymerases.
Mutation Detection: Used in assays where the selective incorporation of dGTP can help identify point mutations or sequence variations.
DNA Repair Assays: Involved in experiments studying DNA repair mechanisms where dGTP plays a role in replication fidelity.
Product Name: 2′-Deoxyguanosine-5′-triphosphate, sodium salt solution
Molecular Formula:C10H13N5Na3O13P3
Molecular Weight: 573.1
CAS Number: 93919-41-6
| TEST | SPECIFICATION |
| Appearance | Clear colorless solution |
| Concentration | 100mM±3% |
| Purity (HPLC area%) | ≥99% |
| λmax | 253nm±2nm |
| Absorbance Rations | A250/A260 1.11~1.19 |
| (pH 7.0) | A280/A260 0.63~0.69 |
| pH (22~25°C) | 7.0±0.1 |
Quality control
1.The concentration is verified by optical density spectrophotometry.
2.This preparation is free of DNase and RNase contamination.
3.The purity of this preparation is determined by HPLC. The minimum passing specification for the triphosphate content is 99%.
4.This lot of dGTP has been performance tested with Taq DNA polymerase and Pfu DNA polymerase.
5.This lot of dGTP has performance tested with Q-PCR.
Storage Recommendations
Long term (Infrequent use; 1-2 times per month): -70°C
Daily/Weekly use: -20°C
Always avoid freeze-thaw cycles or exposure to frequent temperature changes.
These fluctuations can greatly alter product stability.
Standard package size
1ml/vial
10ml/vial
25ml/bottle
50ml/bottle
Other package sizes are available
Delivery Time
2 working days
