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dCTP 100mM solution-Reliable 2′-Deoxyadenosine-5′-triphosphate, sodium salt solution
- Introduction
Introduction
dCTP 100mM solution Ultra pure HPLC>99%
Description
Introducing the dCTP 100mM Solution, a superior quality and ultra-pure 2′-Deoxyadenosine-5′-triphosphate (dCTP), sodium salt formulation. This ready-to-use reagent is meticulously prepared at a concentration of 100mM to provide unwavering reliability in molecular biology applications where authentic nucleotide substrates are essential.
Applications:
Polymerase Chain Reaction (PCR): A critical component in DNA amplification, providing the cytosine base necessary for accurate primer extension during PCR cycles.
DNA Sequencing: One of the four deoxynucleotide triphosphates required for Sanger sequencing and next-generation sequencing methodologies.
Real-Time Quantitative PCR (qPCR): Ensures precise quantification of target DNA sequences by providing the necessary dCTP for template-dependent amplification.
DNA Labeling: In radioisotope or fluorescent labeling reactions where dCTP can be modified for microarray experiments or other detection methods.
Gene Synthesis and Cloning: As a key substrate for in vitro gene assembly and cloning procedures that involve DNA polymerases.
Mutation Detection: Involved in assays where specific incorporation of dCTP helps identify mismatches or mutations in DNA samples.
The dCTP 100mM Solution from [Manufacturer's Name] serves as a cornerstone for researchers who demand reliability and excellence in their nucleic acid synthesis and manipulation endeavors.
Product Name: 2′-Deoxycytidine-5′-triphosphate, sodium salt solution
Molecular Formula:C9H13N3Na3O13P3
Molecular Weight: 533.1
CAS Number: 102783-51-7
| TEST | SPECIFICATION |
| Appearance | Clear colorless solution |
| Concentration | 100mM±3% |
| Purity (HPLC area%) | ≥99% |
| λmax | 272nm±2nm |
| Absorbance Rations | A250/A260 0.79~0.85 |
| (pH 7.0) | A280/A260 0.93~1.03 |
| pH (22~25°C) | 7.0±0.1 |
Quality control
1.The concentration is verified by optical density spectrophotometry.
2.This preparation is free of DNase and RNase contamination.
3.The purity of this preparation is determined by HPLC. The minimum passing specification for the triphosphate content is 99%.
4.This lot of dCTP has been performance tested with Taq DNA polymerase and Pfu DNA polymerase.
5.This lot of dCTP has performance tested with Q-PCR.
Storage Recommendations
Long term (Infrequent use; 1-2 times per month): -70°C
Daily/Weekly use: -20°C
Always avoid freeze-thaw cycles or exposure to frequent temperature changes.
These fluctuations can greatly alter product stability.
Standard package size
1ml/vial
10ml/vial
25ml/bottle
50ml/bottle
Other package sizes are available
Delivery Time
2 working days
