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dTTP 100mM solution-Reliable 2′-Deoxythymidine-5′-triphosphate, sodium salt solution
- Introduction
Introduction
dTTP 100mM solution Ultra pure HPLC>99%
Description
Introducing the dTTP 100mM Solution, a high-quality and ultra-pure 2′-Deoxythymidine-5′-triphosphate (dTTP), sodium salt formulation. This ready-to-use reagent is precisely prepared at a concentration of 100mM for seamless integration into molecular biology applications that require authentic and reliable nucleotide substrates.
Applications:
Polymerase Chain Reaction (PCR): Essential component for DNA synthesis during PCR, providing the thymine base required for accurate replication.
DNA Sequencing: As one of the four deoxynucleotide triphosphates needed for automated Sanger sequencing and next-generation sequencing methods.
DNA Labeling and Nick Translation: Used in labeling reactions where dTTP can be chemically modified with fluorescent tags or biotin for microarray hybridization or detection purposes.
Real-Time qPCR: For precise quantification of DNA targets, where high-quality dTTP ensures accuracy in data interpretation.
Gene Synthesis and Cloning: Critical substrate for in vitro gene synthesis and cloning experiments involving DNA polymerases.
Mutation Analysis: In combination with specialized enzymes, dTTP incorporation can aid in mismatch analysis and mutation detection assays.
Product Name: 2′-Deoxythymidine-5′-triphosphate, sodium salt solution
Molecular Formula:C10H14N2Na3O14P3
Molecular Weight: 548.1
CAS Number: 18423-43-3
| TEST | SPECIFICATION |
| Appearance | Clear colorless solution |
| Concentration | 100mM±3% |
| Purity (HPLC area%) | ≥99% |
| λmax | 267nm±2nm |
| Absorbance Rations | A250/A260 0.62~0.68 |
| (pH 7.0) | A280/A260 0.69~0.79 |
| pH (22~25°C) | 7.0±0.1 |
Quality control
1.The concentration is verified by optical density spectrophotometry.
2.This preparation is free of DNase and RNase contamination.
3.The purity of this preparation is determined by HPLC. The minimum passing specification for the triphosphate content is 99%.
4.This lot of dTTP has been performance tested with Taq DNA polymerase and Pfu DNA polymerase.
5.This lot of dTTP has performance tested with Q-PCR.
Storage Recommendations
Long term (Infrequent use; 1-2 times per month): -70°C
Daily/Weekly use: -20°C
Always avoid freeze-thaw cycles or exposure to frequent temperature changes.
These fluctuations can greatly alter product stability.
Standard package size
1ml/vial
10ml/vial
25ml/bottle
50ml/bottle
Other package sizes are available
Delivery Time
2 working days
