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What is m-mlv reverse transcriptase?

What is m-mlv reverse transcriptase?

M-MLV reverse transcriptase is a type of RNA-dependent DNA polymerase called Moloney Murine Leukemia Virus Reverse Transcriptase which serves as a critical element in the field of molecular biology and genetic research. This enzyme is able to synthesize a complementary DNA strand using long mRNA templates that may exceed 5 kilobases in length. It is produced from the pol gene of the Moloney murine leukemia virus and consists of only one subunit with an approximate weight of 71kDa.

The uses for M-MLV reverse transcriptase are vast, ranging from basic science investigations to clinical diagnostics. By virtue of its capacity to turn RNA into DNA, this enzyme allows scientists to study gene expression, clone expressed genes and detect RNA molecules. Additionally, because it has lesser RNase H activity than most other similar enzymes do, this means cDNA synthesis is facilitated more efficiently thus making it widely applicable across many different molecular techniques.

Within the framework of modern biotechnology. protein engineering methods have been employed on M-MLV reverse transcriptase resulting into improved versions or mutants having superior properties. These modified forms were created with specific objectives in mind i.e., overcoming certain limitations associated with the original M-MLV RT like increased thermostability or reduced RNase H activity. thereby broadening its range of application in various molecular diagnostic methods as well as genome editing approaches.

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Tinzyme Co., Limited was founded in 2015,  is a professional molecular bio reagent Manufacturer in China. The key products are nucleic acid substances and the enzymes for molecular biology. We have many patents for the nucleic acid and the enzymes. And the monomers of the nucleic are all enzymatically synthesized using our proprietary unique technology. These dNTPs and NTPs are functionally approved for all PCR and related reactions including Real Time PCR, in vitro transcription assays and DNA sequencing in diagnostics and molecular biology. Also the enzymes such as Taq DNA polymerase, M-MLV Reverse Transcriptase, pfu DNA polymerase are all worked very well. The performance of our products is comparable to the top bio companies of the world.

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FREQUENTLY ASKED QUESTION

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What is m-mlv reverse transcriptase and how does it work?

M-MLV reverse transcriptase is an enzyme used in molecular biology to convert RNA into DNA. It works by catalyzing the synthesis of a complementary DNA strand from an RNA template, which is essential for various applications such as cDNA library construction, RT-qPCR, and RNA research.

What are the main applications of m-mlv reverse transcriptase?

M-MLV reverse transcriptase is primarily used in reverse transcription reactions, which are crucial for applications like gene expression analysis (RT-qPCR), cDNA synthesis, and RNA virus research. It's also used in cloning experiments and RNA sequencing.

Is m-mlv reverse transcriptase compatible with all types of RNA?

Yes, m-MLV reverse transcriptase is designed to work with a wide range of RNA substrates, including mRNA, tRNA, rRNA, and viral RNA. However, it's always recommended to check the manufacturer's guidelines for specific RNA types.

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