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T4 Gene 32 Single-stranded DNA Binding Protein (SSBP) - High-affinity SSB for Enhanced Nucleic Acid Manipulation in PCR, Cloning, and DNA Repair Assays
- Introduction
Introduction
Description
Introducing our T4 Gene 32 Single-stranded DNA Binding Protein (SSBP), a high-performance reagent that harnesses the exceptional single-strand DNA binding properties of the T4 bacteriophage gene 32 product. This recombinant SSBP is renowned for its extraordinary affinity and specificity towards ssDNA, which significantly improves the efficiency and stability in various nucleic acid manipulation techniques such as PCR, cloning, and DNA repair assays.
Applications
RT-PCR & cDNA Synthesis
Features
PCR Optimization: Enhances primer annealing and extension rates during PCR amplification, especially beneficial for challenging templates with high GC content or long amplicons.
Molecular Cloning: Improves cloning success rates by stabilizing ssDNA during restriction digestion, ligation, and transformation, leading to higher numbers of correctly assembled clones.
DNA Repair Assays: Supports accurate monitoring and manipulation of ssDNA regions during DNA repair pathways, including base excision repair, nucleotide excision repair, and homologous recombination studies.
Sequencing Library Preparation: Ensures stable ssDNA during adapter ligation and library amplification steps for next-generation sequencing workflows.
Gene Assembly Techniques: Facilitates seamless assembly of synthetic genes and genome editing projects by maintaining ssDNA intermediates in vitro.
Nucleic Acid Manipulation: Essential for any experiment involving the handling and processing of ssDNA, such as nick translation, primer extension, and rolling circle amplification.
The T4 Gene 32 Single-stranded DNA Binding Protein serves as an indispensable component in molecular biology laboratories worldwide, empowering researchers with a reliable and optimized solution for manipulating nucleic acids in complex biological systems. Its advanced properties ensure reproducibility and enhanced performance in even the most demanding experimental conditions.
Transportation
on blue ice
Storage
at -20°C for 24 months
