Ribonuclease R, also known as RNase R, is an important enzyme that participates in RNA metabolism throughout all domains of life. It is a member of the RNase II family and acts as a 3’ exoribonuclease, meaning it breaks down RNA molecules from their 3’ end to the 5’ end. This nuclease stands out because it can degrade highly structured RNAs, which other exoribonucleases fail to do. The fact that it is found everywhere and works fast when destroying RNA shows how much impact it has on different biological processes like mRNA decay or rRNA maturation.
One thing about RNase R that makes it special is its ability to unwind and break down duplex RNA with a 3' overhang. therefore, there may be another function for this enzyme besides being an exoribonuclease – it might have some kind of activity related to unwinding RNA secondary structures. These enzymes are often problematic for other proteins since they have complicated secondary structures themselves hence making such compounds difficult targets for enzymes like these ones. Its inherent ATP-dependent helicase activity against dsRNAs at low temperatures where rna structures are stable lower than usual plays a key role in its role as ribonuclease especially with double stranded rna substrates hence acting as catalysts for rnases.
In practice, researchers can take advantage of RNase Rs unique ability to recognize and digest circular RNAs (circRNAs) when conducting molecular biology experiments. Circular or lariat-shaped molecules remain largely unaffected by its action while linear ones get broken up. thus this reagent finds wide application in studies focused on gene expression or alternative splicing where researchers need to remove linear versions of transcripts but keep those that have undergone such processing events intact. In sequencing-based methods designed for circRNA discovery where removing linears is necessary prior accurate circRNA detection/analysis via high-throughput sequencing technologies.
Tinzyme Co., Limited was founded in 2015, is a professional molecular bio reagent Manufacturer in China. The key products are nucleic acid substances and the enzymes for molecular biology. We have many patents for the nucleic acid and the enzymes. And the monomers of the nucleic are all enzymatically synthesized using our proprietary unique technology. These dNTPs and NTPs are functionally approved for all PCR and related reactions including Real Time PCR, in vitro transcription assays and DNA sequencing in diagnostics and molecular biology. Also the enzymes such as Taq DNA polymerase, M-MLV Reverse Transcriptase, pfu DNA polymerase are all worked very well. The performance of our products is comparable to the top bio companies of the world.
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RNase R is an exoribonuclease enzyme that specifically degrades RNA. It is commonly used in molecular biology applications to remove RNA contaminants from DNA samples, ensuring the purity of the DNA for further analysis or manipulation.
Yes, RNase R is designed to be compatible with a wide range of RNA degradation protocols, making it a versatile tool for researchers working with different experimental setups.
RNase R is primarily used for in vitro applications, such as RNA sample preparation and purification. However, it can also be applied in certain in vivo studies where RNA degradation is required.
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