Proteinase K Solution, 20mg/ml, Room Temperature Stable, High Purity, Broad-Spectrum Serine Protease for DNA/RNA Extraction & Protein Degradation in Biological Samples, Enhanced Stability and Consistent Performance

Description

Proteinase K Solution, 20mg/mL Our premium Proteinase K Solution offers a highly concentrated and pure form of the broad-spectrum serine protease enzyme. With a concentration of 20 milligrams per milliliter, this stable formulation is designed to maintain its enzymatic activity even at room temperature, ensuring convenient handling and storage.

This solution not only exhibits remarkable enzymatic activity but is also formulated to maintain its potency even under ambient conditions, providing researchers with unparalleled convenience in handling and storing this essential reagent.

Storage Conditions

Daily/Weekly use: Store vial at RT

For long term storage (Infrequent use; 1-2 times per month): 2-4°C

Expiration date three year of powder and 12 months of sterile liquid

Storage Buffer: 20 mM Tris-HCl (pH 7.4), 1 mM CaCl2, 50% Glycerol (Dilution buffer without glycerol).

QC Assays

• Unit Definition: One unit is defined as the amount of enzyme that will liberate 1 μmol of tyrosine per minute at 37ºC, pH7.5.

• DNase Activity: None detectable enzyme activity with λ DNA after 6 hrs incubation at 37ºC.

• RNase Activity: None detectable ribonuclease activity after 16 hrs incubation at 25ºC.

Applications

Proteinase K is a broad substrate non-specific serine proteinase. It is very stable at pH 4-12. It was used

on isolating mRNA, genomic DNA and digesting unwanted proteins during DNA and RNA preparations

from different kinds of cells. It’s been used on glycoprotein modification and protein structure studies

also. Proteinase K is active with SDS, urea and EDTA.

Proteinase K is used for the isolation of native high molecular genomic nucleic acids. Enzymes like

DNases and RNases from microorganisms and mammalian cells are rapidly inactivated by Proteinase K.

Adding Proteinase K already during the cell lysis enables the isolation of highly native undamaged high

molecular DNA or RNA. A variety of methods have been established, which are documented in

numerous publications.

Recently, Proteinase K has been used for the detection of BSE forming proteins which are uniquely

resistant towards the enzyme’s proteolytic cleavage.

Proteinase K is very useful in the analysis of membrane structure by means of modification of proteins

and glycoproteins on cell surfaces.

Because of the cleavage specificity Proteinase K, characteristic fragments of proteins are obtained which

are helpful in revealing the structure and function of proteins, particularly enzymes. 

Its compatibility with substances like SDS, urea, and EDTA further enhances its utility in a wide range of

experimental conditions.

Other Names

• Proteinase K

• endopeptidase K

• Tritirachium alkaline proteinase

• Tritirachium album proteinase K

• Tritirachium album serine proteinase