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What is T7 RNA polymerase?

What is T7 RNA polymerase?

The T7 RNA polymerase (T7 RNAP) is an enzyme with high efficiency and specificity, derived from the bacteriophage T7. It helps in transcribing nucleic acids by converting DNA sequences into RNA molecules. The excellence of this enzyme lies in its ability to recognize and attach itself to the T7 phage promoters during initiation of transcription. Although it is a single-subunit enzyme with a molecular weight of about 98,000 Da; T7 RNAP shares some homology with DNA polymerase I family of polymerases but differs structurally from multisubunit RNA polymerases found in eukaryotes.

In the transcription process, T7 RNAP attaches itself specifically to promoter DNA starting from nucleotide position -17 through −5. From position −4 to +3, it melts down the double-stranded structure of DNA thereby enabling RNA synthesis initiation. Processivity of the protein allows it transcribe an entire gene without other proteins’ help thus making it widely used for in vitro RNA synthesis. The fact that T7 RNAP can start transcription at any specific site by binding a 17-base-pair promoter DNA makes different from other RNA polymers.

Many researchers have come to appreciate T7 RNAP because of its efficiency and specificity which has made it very important tool in molecular biology and biotechnology applications such as cloning where one needs produce any length RNAS easily thru overexpressing them using cloning vectors containing inducible tetracycline operator sites inserted downstream region under control lacUV5 promoter sequence cloned into pET vector etc.. Another use would be synthesis mRNA s for research or commercial purposes since full-length transcripts are produced when using this type rna polyermase structural studies on t- Seven Pico have helped us understand how these enzymes work better during transcriptions so far

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Tinzyme Co., Limited was founded in 2015,  is a professional molecular bio reagent Manufacturer in China. The key products are nucleic acid substances and the enzymes for molecular biology. We have many patents for the nucleic acid and the enzymes. And the monomers of the nucleic are all enzymatically synthesized using our proprietary unique technology. These dNTPs and NTPs are functionally approved for all PCR and related reactions including Real Time PCR, in vitro transcription assays and DNA sequencing in diagnostics and molecular biology. Also the enzymes such as Taq DNA polymerase, M-MLV Reverse Transcriptase, pfu DNA polymerase are all worked very well. The performance of our products is comparable to the top bio companies of the world.

Why Choose Tinzyme

Unmatched Efficiency

At TINZYME, our Poly A RNA carriers excel in unmatched efficiency, ensuring swift and effective delivery of RNA molecules to their target destinations within cells. This efficiency is pivotal for maximizing therapeutic outcomes and minimizing potential side effects.

Superior Stability

The superior stability of TINZYME Poly A RNA carriers is a cornerstone of their effectiveness. Our carriers protect RNA from degradation, extending its therapeutic window and enhancing the overall potency of RNA-based treatments.

Versatile Compatibility

TINZYME Poly A RNA carriers boast versatile compatibility, making them suitable for a wide array of RNA types and therapeutic applications. This adaptability empowers researchers and clinicians with a flexible platform for innovative RNA-based therapies.

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FREQUENTLY ASKED QUESTION

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What is t7 rna polymerase and what does it do?

T7 RNA polymerase is an enzyme that is responsible for synthesizing RNA from a DNA template. It is commonly used in molecular biology research for in vitro transcription reactions, allowing researchers to produce large quantities of specific RNA molecules for various applications such as RNA interference, ribozymes, and RNA structural studies.

How does t7 rna polymerase differ from other RNA polymerases?

T7 RNA polymerase is unique in its ability to initiate transcription without the need for a promoter sequence within the host cell's genome. This feature makes it highly efficient and specific for in vitro transcription reactions, as it can transcribe any DNA template provided to it. Additionally, T7 RNA polymerase has a high processivity, meaning it can synthesize long RNA strands without dissociating from the DNA template.

Is t7 rna polymerase safe to handle in a laboratory setting?

Yes, T7 RNA polymerase is generally considered safe when handled according to standard laboratory protocols. However, like any laboratory reagent, it should be treated with respect and appropriate safety measures should be followed, including wearing gloves and working in a biological safety cabinet if necessary.

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