Taq DNA Polymerase Bulk Pack - High Fidelity, Robust Thermal Stability, Process-Optimized Enzyme Solution for Enhanced PCR Amplification Efficiency and Reliable RT-PCR Performance in High Throughput Applications, Ideal for Genotyping, Cloning, Library Pre

Description

Taq DNA Polymerase Isolated from the E. coli cloning Thermus aquaticus. The molecular weight

is approximately 94 kDa. EasyTaq DNA polymerase has the 5′ to 3′ DNA polymerase activity and 5′ to 3′

exonuclease activity without 3′ -5′ exonuclease activity. The extending speed is 1-2 kb/min. There is an “A” on 3′ end. The PCR product can be cloned in TA vector.

Conc. 5 U/μl

Store at -20°C

Key Features

• Highly Concentrated Bulk Format: Sufficient to support large-scale PCR runs and multiple experimental setups.

• Superior Thermal Stability: Withstands repeated cycles of denaturation, annealing, and extension without losing activity, ensuring robust amplification over a broad temperature range.

• High Fidelity PCR: Minimizes error rates during DNA synthesis, resulting in accurate amplicons for downstream applications such as sequencing and cloning.

• Efficient RT-PCR Performance: Proven effectiveness in synthesizing cDNA from RNA templates, ideal for gene expression analysis and viral detection.

• Versatile Use: Suitable for diverse molecular techniques including genotyping, cloning, next-generation sequencing library preparation, and diagnostic tests.

• The Taq DNA Polymerase bulk pack is designed with the end-user's needs in mind, providing both the reliability demanded by routine laboratory protocols and the scalability required for high-throughput research and industrial settings.

Characteristics

1.high-sensitivity

2.high amplification efficiency

Unit Definition

One unit of Platinum Taq DNA Polymerase High Fidelity incorporates 10 nmol of deoxyribonucleotide

into acid-precipitable material in 30 minutes at 74°C.

Quality Control

• functional absence of double- and single-stranded endonuclease activity;

• Purity>90% homogeneous by SDS gel electrophoresis.

• Each lot of EasyTaq DNA Polymerase is assayed for amplification from as little as 10 ng of human

genomic DNA.

Storage Buffer

20 mM Tris-HCl (pH 8.0),0.1 mM EDTA,1 mM DTT,100 mM KCl,Stabilizers,50% glycerol.

Reaction Mixture Set Up

Recommended thermal cycling conditions

94°C 2-5 min

94°C 30 sec

50-60°C 30 sec 30-35 cycles

72°C 1-2 kb/min

72°C 5-10 min

Package Details

Bulk package Store at -20 degree With low temperature shipping