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dATP 100mM solution-Reliable 2′-Deoxyadenosine-5′-triphosphate, sodium salt solution
- Introduction
Introduction
Description
Introducing our dATP 100mM solution, a premium quality reagent formulated with high purity 2′-Deoxyadenosine-5′-triphosphate sodium salt. This ready-to-use solution offers researchers a reliable source of deoxyadenosine triphosphate for various molecular biology and biochemical applications.
Product Name: 2′-Deoxyadenosine-5′-triphosphate, sodium salt solution
Molecular Formula:C10H13N5Na3O12P3
Molecular Weight: 557.2
CAS Number: 1927-31-7
| TEST | SPECIFICATION |
| Appearance | Clear colorless solution |
| Concentration | 100mM±3% |
| Purity (HPLC area%) | ≥99% |
| λmax | 259nm±2nm |
| Absorbance Rations | A250/A260 0.75~0.81 |
| (pH 7.0) | A280/A260 0.13~0.18 |
| pH (22~25°C) | 7.0±0.1 |
Key Features
High Concentration: The solution is prepared at a concentration of 100mM, providing an ample supply of dATP for multiple reactions without the need for dilution.
Superior Purity: With a purity specification of over 99%, as determined by HPLC analysis (C18 column), this dATP solution ensures low contamination levels, leading to more accurate and reproducible results.
Stable Storage: Packaged in a manner that maintains stability, the dATP solution should be stored at -20°C to guarantee long-term preservation of its activity.
Manufacturer Quality: Offered by reputable brands such as Yaoyue or Baiolaibo, our dATP product is trusted by professionals worldwide for its consistent performance.
Versatile Use: Specially designed for compatibility with sensitive procedures like PCR (polymerase chain reaction) and other nucleic acid synthesis methods.
Applications
PCR Amplification: Used as a critical energy source and building block in DNA polymerization reactions during PCR amplification processes.
DNA Sequencing: Essential component in DNA sequencing reactions where it serves as a substrate for DNA polymerases.
Nucleotide Labeling: For labeling nucleic acids with radioisotopes or fluorescent tags in molecular diagnostics and research assays.
Gene Cloning: Facilitating the construction of recombinant DNA molecules by supplying energy and nucleotides required for enzymatic ligation.
cDNA Synthesis: Employed in reverse transcription reactions to synthesize complementary DNA from RNA templates.
This dATP 100mM solution is an indispensable tool for laboratories engaged in cutting-edge biological research and diagnostic applications requiring precision and reliability.
Quality control
1.The concentration is verified by optical density spectrophotometry.
2.This preparation is free of DNase and RNase contamination.
3.The purity of this preparation is determined by HPLC. The minimum passing specification for the triphosphate content is 99%.
4.This lot of dATP has been performance tested with Taq DNA polymerase and Pfu DNA polymerase.
5.This lot of dATP has performance tested with Q-PCR.
Storage Recommendations
Long term (Infrequent use; 1-2 times per month): -70°C
Daily/Weekly use: -20°C
Always avoid freeze-thaw cycles or exposure to frequent temperature changes.
These fluctuations can greatly alter product stability.
Standard package size
1ml/vial
10ml/vial
25ml/bottle
50ml/bottle
Other package sizes are available
Delivery Time
2 working days
