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N1-Me-Pseudo UTP 100mM Solution-Reliable N1-Me-Pseudouridine-5′-Triphosphate trisodium salt solution
- Introduction
Introduction
Description
Product Description: Introducing our N1-Me-Pseudo UTP 100mM Solution, a high-quality and reliable source of N1-methyl-pseudouridine-5′-triphosphate trisodium salt. This modified nucleotide is a key component for enhancing the performance and biostability of synthetic mRNA molecules in vitro and in vivo applications.
Key Features
Advanced Nucleoside Modification: N1-Me-Pseudo UTP is a derivative of pseudouridine, with a methyl group at the N1 position, reducing immunogenicity and improving mRNA translational efficiency.
High Purity Formula: The solution contains ≥99% pure N1-methyl-pseudouridine-5′-triphosphate trisodium salt, ensuring minimal contamination and optimized incorporation into RNA transcripts.
Convenient Concentration: Supplied as a ready-to-use 100mM solution, providing an exact concentration for efficient transcription reactions without the need for laborious dilutions.
Stable Storage: Packaged to maintain stability when stored at -20°C, avoiding freeze-thaw cycles to preserve activity and integrity.
Versatile Use: Compatible with various in vitro transcription systems, enabling the synthesis of chemically modified mRNAs that mimic endogenous cellular mRNA properties.
Applications
mRNA Therapeutics: Ideal for use in the production of modified mRNA vaccines and therapeutics where reduced immune recognition and improved translation are critical.
Gene Expression Studies: Employed in the preparation of functional, capped, and polyadenylated mRNAs for transfection experiments or delivery into cells for controlled gene expression.
Synthetic Biology: Used in the creation of custom-designed mRNA constructs with enhanced stability and biological function for research purposes.
Protein Production: Facilitates increased protein yield from transfected mRNA by enhancing ribosome binding and translation rates.
In Vitro Translation Systems: Essential for optimizing mRNA templates used in cell-free protein synthesis platforms to improve reaction yields.
The N1-Me-Pseudo UTP 100mM Solution provides researchers and pharmaceutical developers with a premium reagent to produce next-generation modified mRNAs, thereby advancing their therapeutic potential and expanding the possibilities within the field of molecular biology and medicine.
Product Name
N1-Me-Pseudo-UTP, 100mM Solution
(N1-Me-Pseudouridine-5′-Triphosphate trisodium salt solution)
CAS No.
1428903-59-6
Molecular Formula
C10H14N2Na3O15P3
Molecular Weight
564.11
Molar absorptivity
8500 (λ=271nm)
Specification
| Appearance | Clear colorless solution |
| Purity(by HPLC) | ≥99.0% |
| λmax | 272nm±2nm |
| Concentration | 100mM±3 |
| 31P NMR(D2O) | To conform structure |
| 1H NMR(D2O) | To conform structure |
| pH(in buffer solution) | 7.0±0.1 |
Storage Recommendation
Please store in -20°C. Always avoid freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.
Quality Control
1. The concentration is verified by Uv-vis spectrophotometry.
2. This lot of N1-Me-Pseudo-UTP is free of DNase and RNase contamination.
3. The purity of this preparation is determined by HPLC.
