Premium-Grade M-MLV Reverse,Transcriptase, High quality bulk pack for IVD

Description

Introducing our exclusive M-MLV Reverse Transcriptase in a high-quality bulk pack, tailored for In Vitro Diagnostic (IVD) use. This enzyme stands apart from the competition, offering unparalleled reliability and efficiency in cDNA synthesis from RNA templates. Ideal for molecular biology laboratories seeking precision and scalability in their research endeavors.

Source

Recombination of E. coli containing Moloney murine leukemia virus reverse transcriptase gene from clone of Moloney murine.

Applications

• cDNA Synthesis: Vital for converting total RNA or mRNA into complementary DNA (cDNA) as a prerequisite step for quantitative PCR (qPCR), RT-qPCR, and other gene expression analysis methods.

• Gene Cloning: Used to clone cDNA sequences into plasmids for recombinant protein expression or functional genomic studies.

• RNA Profiling: Essential in microarray experiments and next-generation sequencing (NGS) library preparation workflows for transcriptome analysis.

• Diagnostic Assays: Integral part of IVD kits that require the conversion of RNA targets to DNA for detection, such as infectious disease diagnostics and genetic testing.

• mRNA Characterization: Facilitates the study of mRNA structure, splicing variants, and post-transcriptional modifications by providing stable cDNA intermediates.

• M-MLV Reverse Transcriptase in bulk pack: Trusted for routine & complex diagnostics, delivering consistent results & scalability in demanding environments.

Features

• lack RNase H activity: Weak RNase H activity High cDNA yield, can get more full length cDNA.

• thermoStable: the optimum reaction temperature is 50°C, the highest is 60°C. Can overcome the template RNA secondary structure, and finish the reverse transcriptase experiment smoothly.

• wide temperature range: can reverse transcript from 37-60C, with more than 80% of the highest activity at 42°C-55°C customer can choose the reaction temperature freely.

• Strong amplification activity: Through genetic mutation, the enzyme's binding capacity with RNA has been significantly enhanced. This increase in amplification speed enables the production of high-quality cDNA, making it an excellent choice for cDNA library construction and other demanding molecular biology applications.

Unit Definition

One unit of MMLV RT catalyzes the incorporation of 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using oligo(dT)12-18-primed poly(A)n as a template.

Concentration: 200U/μl

Package: Bulk

Component: M-MLV (200U/μl) 5xBuffer (with DTT)

Storage: -20°C