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MTaq DNA Polymerase - High-Performance Blood Direct PCR Reagent for Rapid and Efficient Direct Amplification
- Introduction
Introduction
Description
Introducing the cutting-edge mTaq DNA Polymerase - Blood Direct PCR Reagent, an innovative reagent that revolutionizes the amplification of DNA from whole blood samples. Unlike traditional methods that require laborious purification steps, this reagent directly amplifies DNA from blood lysates, significantly reducing sample preparation time and enhancing the overall efficiency of the PCR workflow. Its unique formulation ensures highly specific and robust amplification, making it an indispensable tool for rapid and accurate genetic analysis.
Key Features:
Blood-Derived DNA Amplification Unhindered: The mTaq DNA Polymerase - Blood Direct PCR Reagent is specifically engineered to withstand the inhibitory effects of heme and other common blood components. This unique tolerance allows for seamless PCR reactions, overcoming the traditional challenges associated with amplifying DNA from blood samples.
Direct PCR Capability: Eliminates laborious DNA extraction steps, allowing researchers to perform PCR directly on crude blood samples, saving time and resources while reducing contamination risks.
Enhanced Sensitivity & Unparalleled Specificity: The mTaq DNA Polymerase - Blood Direct PCR Reagent excels in its ability to precisely target desired sequences, even in challenging biological matrices. Its enhanced sensitivity ensures reliable amplification of even long templates, reaching lengths of several kilobases, without compromising on specificity. This unique combination of sensitivity and specificity sets it apart from other reagents, delivering highly accurate and consistent results.
Hot Start Variant: Incorporates hot start technology that prevents premature extension during reaction setup, ensuring improved primer annealing and increased yield of desired PCR products.
Robust Performance: Demonstrates consistent performance across a wide range of template dilutions and varying blood volumes, making it suitable for a broad spectrum of applications and experimental conditions.
Ease of Use Unmatched: The mTaq DNA Polymerase - Blood Direct PCR Reagent comes as a ready-to-use master mix, eliminating the need for manual buffer preparation or dNTP mixing. This streamlined format not only simplifies PCR setup but also significantly reduces pipetting errors, ensuring consistent and reliable results across experiments.
Applications:
Molecular Diagnostics: Ideal for rapid detection of genetic markers or infectious agents directly from patient blood samples in clinical diagnostic settings.
Forensic Analysis: Streamlines the process of generating DNA profiles from crime scene stains containing blood, enhancing efficiency in forensic laboratories.
Genetic Screening: Enables quick and cost-effective screening of blood samples for genetic diseases, gene mutations, or polymorphisms.
Transcriptomic Studies: Facilitates expression analysis by cDNA synthesis and subsequent PCR amplification from RNA present in whole blood.
Population Genetics: Supports large-scale genotyping projects where processing large numbers of blood samples is necessary.
Custom Assays: Can be tailored for use in custom-designed PCR assays for research purposes, including SNP genotyping, fragment analysis, and cloning.
Concentration: 5 u/µl
Package: Bulk
Storage: Store at -20°C
Unit Definition
One unit of mTaq DNA Polymerase High Fidelity incorporates 10 nmol of deoxyribonucleotide into
acid-precipitable material in 30 minutes at 74°C.
Features
Can directly use for amplification frozen or fresh blood, template do not need pretreatment or purification.
Directly amplify the template of dried blood and interstitial fluid.
Amplify the DNA from the crude template such as feces and soil extractive.
The amplification speed is twice of the general Taq DNA Polymerase.
Quality control
Functional absence of double and single-stranded endonuclease activity;
Purity>99% test by SDS gel electrophoresis;
Each lot of mTaq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA;
Retain full activity at room temperature for one week;
No host DNA residue.
Application
Crude template DNA amplification.
Genotyping
complex DNA template amplification.
High throughput genetic analysis.
