Rnase Inhibitor: Protecting Rna Integrity In Laboratory Experiments

RNA is of utmost importance when one works in the field of molecular biology, especially during experiments that would require the RNA to be intact. An RNase inhibitor is a critical tool that is used to protect RNA from degradation in order to avoid inaccuracies in various applications enlisted.

RNases Explained

Ribonuclease (RNase) is an enzyme whose function is to degrade RNA, something that is quite common in laboratory settings. There are various sources of contamination that in most cases introduce RNases into the RNA that is collected, and these include skin, equipment, and even air; hence a huge fraction of RNA quality is lost. Those processes that require RT-PCR, cloning, and sequencing which are known for their high requirements in RNA integrity would suffer because of this degradation.

RNase Inhibitors’ Description

RNase inhibitors are specific types of proteins aimed at binding RNase molecules in order to protect material RNA from degradation. By employing such inhibitors, the researchers are able to establish an ambiance of their choice that does not involve the disintegration of RNA improving the accuracy of such experiments. None of these inhibitors list placental RNase inhibitor derived from the source.

Research Implications

The use of RNase inhibitors is quite broad and extends to the areas of molecular biology and genetics. The use of them is also quite useful in the procedures surrounding the collection of RNA, reverse transcripts, and amplification processes. Such inhibitors do not only make the procedures easier but more useful since the RNA will not be degraded thus more reliable outcomes from experiments will be obtained limiting or eliminating the chances of errors stemming from RNA degradation.

Important Information Regarding Usage

In order to optimize the efficiency of RNase inhibitors, the correct storage and usage techniques must be employed. Every RNase researcher must carry out their work in RNase-free areas with the use of certified reagents and consumables. Furthermore, the use of RNase inhibitors at the recommended levels during manipulation of the RNA sample can safely prolong the RNA samples’ stability.

In closing, it can be stated that RNase inhibitors are extremely important for the preservation of RNA in laboratory manipulation. The reason is very useful in regard to the complexity of RNA studies