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WellStart BST DNA polymerase★★★
- Introduction
Introduction
Product Description:
Our WellStart BST DNA Polymerase ★★★ is a premium thermostable enzyme designed for superior performance in isothermal amplification techniques. This enzyme is a highly optimized version of the Bacillus stearothermophilus DNA polymerase, offering enhanced efficiency and fidelity for a wide range of applications.
Key Features:
- Highly optimized thermostable enzyme for isothermal amplification
- Exceptional efficiency and fidelity in DNA synthesis
- Suitable for various isothermal amplification methods, including LAMP and RCA
- Reduced non-specific amplification and primer-dimer formation
- Ideal for rapid and sensitive detection of nucleic acids
- Compatible with a broad range of buffer conditions and sample types
Applications:
- Isothermal amplification techniques (LAMP, RCA, etc.)
- Point-of-care testing and rapid diagnostics
- Amplification of DNA for downstream applications
- Educational and research settings for isothermal amplification
- Forensic analysis and environmental monitoring
- Development of portable and user-friendly diagnostic devices
Our WellStart BST DNA Polymerase ★★★ is a top-tier choice for researchers and diagnostic developers seeking to achieve high-quality results in isothermal amplification. Its advanced properties ensure a more targeted and efficient amplification process, making it a reliable and powerful tool for a variety of applications.
ThermoStartTM Bst DNA Polymerase
Description
ThermoStartTM Bst DNA polymerase source from Bacillus stearothermophilus DNA polymerase I. it has no 5'-3' exonuclease activity, but enhanced its 5'-3' DNA polymerase activity and strand replacement activity. After genetic engineering, the enzyme has increased the hot start function, which has no activity below 60 degrees. Compared with wild-type Bst DNA polymerase, the enzyme has greatly improved in amplification speed, yield, salt tolerance and thermal stability, and increased dUTP tolerance. It is very suitable for pollution-proof isothermal amplification reactions, such as lamp.
Source
Recombinant E. coli.
Applications
Isothermal DNA amplification and relate research.
Supplied with
10xBst Buffer.
Unit definition
Under 65 °C for 30 min, the amount of enzyme required to add 10 nmol of dNTP into acid insoluble precipitate defined as 1 Unit.
Thermal inactivation
85 °C，5 min.
Storage and transportation
Ship with ice pack, Stored at -20 °C.
Reaction Set up
| Component | Volume（μL） | Final concentration |
| 10×Bst Buffer | 2.5 | 1×(contains 8 mM MgSO4) |
| dNTP Mix (10 mM each) | 3.5 | 1.4 mM each |
| dUTP (100 mM) (optional) | 0.35 | 1.4 mM |
| UDGase (1U/μL) (optional) | 1 | 1 U |
| Template DNA | 10 ng~1 μg | - |
| 10×Primers* | 2.5 | - |
| Thermo Start DNA Polymerase (8 U/μL) | 0.25~1 | 0.08~0.32 U/μL |
| ddH2O | to 25 | - |
Reaction conditions
| Temperature | Time | Effect |
| 25~37 °C | 5~10 min | Degradation of U-containing template (optional) |
| 65 °C | 30~60 min | Reaction |
| 85 °C | 5 min | inactivation |
Thermo Start Bst DNA Polymerase test
COVID-19 N gene was used as a target in the RT-LAMP reaction.
ThermoStart Bst DNA Polymerase, no activity at room temperature. Will be activated at 60°C.
Order
| BS01 | Bst DNA Polymerase | Bst DNA Polymerase, Large Fragment, 8U/μl |
| BS02 | ThermoStart BST DNA Polymerase★★★ | ThermoStart BST DNA Polymerase, Hotstar technology, High specificity and sensitivity, suitable for RT lamp and IVD purpose |