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MMLV Reverse Transcriptase, IVD uchun katta paket - Termostaq M-MLV Reverse Transcriptase(RNase H-)
- Kirish
Kirish
Termal qadrlab obrnatib yozuvchi enzim, RNase H Minus, reaksiya temperaturasi 55°C gacha, 200U/μl
Tavsif
MMLV Reverse Transcriptase, Moloney Murine Leukemia Virus (MMLV RT) tomonidan kodlangan va RNA-ga bog'liq DNA polimerazasi sifatida ishlaydi. Bu enzim, primer hibridlangan bitta jihatli RNA shablonidan komplement cDNA birinchi jihatini sintezi qiladi. MMLV RT shuningdek, bitta jihatli DNA ga hibridlangan primerni uzaytirishi mumkin. Ikkinchi jihatli cDNA ni sintezi ba'zi mRNA shablonlari uchun qo'shimcha DNA polimerazi shart emas. Umumiy M-MLV RT dan farqi shundaki, ushbu enzimning ist yeltirishga qarshi dayomiyligi oshirilgan. U 50°C da 100% faoliyatni saqlaydi va 55°C da ham 80% dan ziyod faoliyatni saqlaydi.
Manba
Moloney murine leukemia virus reverz transcriptaza genini o'z ichiga olgan E. coli ning rekombinatsiyasi Moloney murine klonidan.
Qo'llanish sohaları
Birinchi chorak cDNA sintezi; RT-PCR
Xususiyatlari
- RNase H faoliyati yo'q: Kamchilikda RNase H faoliyati Yuqori cDNA miqdori, to'liq uzunlikdagi cDNA ni ko'proq olish mumkin.
- issiqga qarshilik: optimal reaksiya harorati 50°C, eng yuqori harorat 60°C. Mavzu RNA ikkinchi turdagi strukturani tashlab ketishi mumkin va reverz transcriptaza eksperimentini muvaffaqiyat bilan yakunlashi mumkin.
- keng harorat diapazoni: 37-60C orqali reverz transcript qilish mumkin, eng yuqori faoliyat 42°C-55°C da 80% dan ortiq. Xaridor reaksiya haroratini ozgina tanlashingiz mumkin.
- Kuchli kattalashdirish faoliyati: Gen mutatsiyasi enzym va RNA ning bog'lanish qobiliyatini oshirdi. Kattalashdirish tezligini oshirdi, sifatli cDNA ni olish mumkin, cDNA biblioteka qurish uchun muhim.
Vahidning belgilash usuli
MMLV RT vahidi 37°C da oligo(dT)12-18-primerlangan poly(A)n shablonidan foydalanib 10 daqiqa ichida 1 nmol dTTP ni kattalashgan asiddan pochulmaydigan materialga o'tkazadi.
Mutaxassislik : 200U/μl
Qadoqlash : Bulk
Komponent : M-MLV (200U/μl) 5xBuffer (DTT bilan)
Saqlash : -20°C
buyurtma
| RT01 | m-mlv reversiv transkriptaza (RNase H-) | M-MLV Teskari Transkriptazi, RNase H Minus 200U/μl, 5x Reaksiya Bufferi bilan taqdim etilgan |
| RT02 | M-MLV Teskari Transkriptaza (RNase H+) | M-MLV Teskari Transkriptazi, klassik tur, RNase H musbat. 200U/μl, 5x Reaksiya Bufferi bilan taqdim etilgan. Yuqori faoliyat. |
| RT03 | ThermoStable M-MLV Reverse Transcriptase(RNase H-) | ThermoStable orqalik transcriptaz, RNase H Minus, reaksiya harorati 55°C gacha, 200U/μl |
| RT04 | ThermoSuper M-MLV Reverse Transcriptase(RNase H-) | ThermoSuper stabil orqalik transcriptaz, RNase H Minus, reaksiya harorati 60°C gacha, 200U/μl |
| RT05 | HiFi II M-MLV Reverse Transcriptase(RNase H-) | M-MLV orqalik transcriptaz, RNase H Minus, IVD ishlatish uchun |
| RT05L | HiFi II M-MLV Reverse Transcriptase(RNase H-) Likfilizlangan Toz | M-MLV orqalik transcriptaz, RNase H Minus, IVD ishlatish uchun, lyofilizatsiya tozalari |
| RTY3 | HiFi III M-MLV Reverse Transcriptazasi (RNase H-) | COVID uchun maxsuslashtirilgan M-MLV |
| RT05F | HiFi II M-MLV Oborat Transkriptaza (RNase H-) (Glicerol yo'q) | MMLV |
| RT42 | M-MLV Oborat Transkriptaza (RNase H-) | MMLV Oborat Transkriptaza, 42°C RNase H Minus 200U/μl, 5x Reaksiya Bufferi bilan ta'minlangan |
| IIA16 | HiFi II M-MLV Oborat Transkriptaza (RNase H-) A16 | MMLV Reverz Transcriptaza (RNase H-) IIA16 |