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USP grade Trypsin

  • Introduction
Introduction

Specification Activity:Trypsin>=2500U/mg, Chymotrypsin<=2%; Standard USP43; CAS NO. 9002-07-7

USP grade Trypsin source from Swine / Bovine pancreas

Description

Trypsin is a kind of serine proteolytic enzyme, with molecular weight of 23300 dalton, and is a single peptide chain composed of 223 amino acid residues. With rigorous specificity, the trypsin specifically acts on the peptide linkage constituted by the basic amino acid arginine and leucine. Enzyme easily autolyzes by itself, and its activation also reduces or loses gradually. It is easily soluble in the water, but insoluble in trichloromethane, ethanol, ether and glycerin, and the optimum PH is 8.0~9.0. When the PH is 1.8, it is hardly deactivated when boiled for a short time; if the salt is added into the hot solution, the protein will precipitate, and the enzyme action of filtrate cannot be seen, and Ca2+ plays the role in protecting and activating the trypsin. The high purity Trypsin is purified by re-crystallization, and then by Ion Exchange chromatography and ultra-filtration.

Application

For cell culture, hydrolyze the proteins between cells and disperse them.

Activity ≥ 2500 USP U/mg

Source: Bovine Pancreas

Specification

Items Specification Standard
Appearance White or almost white lyophilized powder USP40
Solubility 500,000USP Trypsin Units is soluble in 10ml of water and in 10ml of saline TS. USP40
Loss on drying No more than 5.0% USP40
Residue on Ignition No more than 2.5% USP40
Microbial limits:
Pseudomonas aeruginosa
Salmonella
Staphylococcus aureus
Absent USP40
Chymotrypsin No more than 50 USP units/mg powder USP40
Assay Trypsin ≥ 2500USP units/mg powder USP40
Description white crystalline powder EP8.0
Identification A) A purple color develops within 3 minutes
B) No purple color develops with in 3 minutes
EP8.0
Appearance of solution Solution S is not more opalescent than reference suspension Ⅲ EP8.0
Absorbance 1) 280nm : 13.5 ~ 16.5
2) 250nm : not more than 7.0
EP8.0
PH 3.0-6.0 EP8.0
Histamine Not more than 1µg/0.2µkatal EP8.0
Loss on drying Not more than 5% EP8.0
Limit of Chymotrypsin Not more than 1% EP8.0
Microbial limits   EP8.0
Total viable aerobic count ≤10000cfu/g  
Salmonella  Absent  
E.Coli Absent  
Assay Not less than 0.5µkatal /mg, calculated on the dried basis. EP8.0

Storage

Store in an airtight container, at a temperature of 2°C to 8°C.

Order

P062001 Trypsin (USP) Specification Activity:Trypsin>=2500U/mg, Chymotrypsin<=2%; Standard USP43; CAS NO. 9002-07-7

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