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Thermo Start Bst DNA Polymerase
- Introduction
Introduction
Product Description:
Our Thermo Start Bst DNA Polymerase is a thermostable enzyme specifically engineered for isothermal amplification techniques. This enzyme is derived from Bacillus stearothermophilus and is designed to initiate DNA synthesis only after reaching the optimal temperature, thus reducing non-specific amplification and primer-dimer formation.
Key Features:
- Thermostable enzyme for isothermal amplification
- Controlled initiation of DNA synthesis at optimal temperature
- Reduced non-specific amplification and primer-dimer formation
- Suitable for isothermal amplification methods such as LAMP and RCA
- High processivity for efficient amplification of long DNA fragments
- Ideal for point-of-care diagnostics and field applications
Applications:
- Isothermal amplification techniques (LAMP, RCA, etc.)
- Point-of-care testing and rapid diagnostics
- Amplification of DNA for downstream applications
- Educational and research settings for isothermal amplification
- Forensic analysis and environmental monitoring
- Development of portable and user-friendly diagnostic devices
Our Thermo Start Bst DNA Polymerase is a valuable asset for researchers and diagnostic developers looking to harness the power of isothermal amplification. Its unique properties enable a more targeted and controlled amplification process, making it a reliable choice for a variety of applications.
Volume（μL） |
Final concentration | |
| 10×Bst Buffer | 2.5 | 1×(contains 8 mM MgSO4) |
| dNTP Mix (10 mM each) | 3.5 | 1.4 mM each |
| dUTP (100 mM) (optional) | 0.35 | 1.4 mM |
| UDGase (1U/μL) (optional) | 1 | 1 U |
| Template DNA | 10 ng~1 μg | - |
| 10×Primers* | 2.5 | - |
| Thermo Start DNA Polymerase (8 U/μL) | 0.25~1 | 0.08~0.32 U/μL |
| ddH2O | to 25 | - |
Reaction conditions
| Temperature | Time | Effect |
| 25~37 °C | 5~10 min | Degradation of U-containing template (optional) |
| 65 °C | 30~60 min | Reaction |
| 85 °C | 5 min | inactivation |
Thermo Start Bst DNA Polymerase test
COVID-19 N gene was used as a target in the RT-LAMP reaction.
ThermoStart Bst DNA Polymerase, no activity at room temperature. Will be activated at 60°C.
Order
| BS01 | Bst DNA Polymerase | Bst DNA Polymerase, Large Fragment, 8U/μl |
| BS02 | ThermoStart BST DNA Polymerase★★★ | ThermoStart BST DNA Polymerase, Hotstar technology, High specificity and sensitivity, suitable for RT lamp and IVD purpose |