Ribonuclease A Recombinant Lyophilized

Product Description:

Our Ribonuclease A Recombinant Lyophilized is a freeze-dried formulation of the high-quality RNA-degrading enzyme produced through recombinant technology. This lyophilized powder format provides enhanced stability and ease of storage, making it an ideal choice for researchers who require a stable and convenient source of Ribonuclease A for their experiments.

Key Features:

• Recombinant technology for high purity and consistent quality
• Lyophilized powder format for long-term stability
• Efficient degradation of RNA molecules
• Suitable for DNA isolation and purification protocols
• Reduced risk of RNA contamination in DNA samples
• Convenient reconstitution for immediate use in various applications

Applications:

• DNA isolation and purification to remove contaminating RNA
• Preparation of DNA samples for PCR and other DNA-based assays
• Elimination of RNA from protein purification processes
• Quality control of DNA samples prior to sequencing or microarray analysis
• Educational and research settings for molecular biology experiments
• Clinical and diagnostic laboratories for DNA sample preparation

Our Ribonuclease A Recombinant Lyophilized is a reliable and user-friendly option for laboratories conducting DNA-related experiments. Its stable lyophilized form ensures consistent performance over time, while its high purity and efficiency guarantee the effective removal of RNA contaminants from DNA samples.

Description

Ribonuclease A (RNase A) is a genetically engineered endonuclease which is originally from bovine genome. The product is produced and purified from yeast cells without contamination of endotoxin.

RNase A is a very stable endoribonuclease and a single chain polypeptide containing four disulfide bridges. Solutions of RNase A have been reported to withstand temperatures up to 100°C. The highest activity is exhibited with single stranded RNA. Activators of RNase A include potassium and sodium salts.

A major application for RNase A is the removal of RNA from preparations of biological products, such as plasmid DNA or protein samples. It is also used in RNA sequence analysis and protection assays.

• CAS No.: 9001-99-4
• E.C.: 3.1.27.5
• Source: Pichia pastoris cells with cloned gene encoding genetically engineered Bovine pancreatic ribonuclease.
• Activity: ≥40 Kunitz units/mg Protein
• Molecular weight: 14.3 kDa
• Form: Lyophilized Powder
• Grade: Molecular biology level
• Purity: ≥ 90%; (SDS-PAGE)
• Endotoxin: None detected (Tachypleus Amebocyte Lysate)
• Foreign activity: Endonuclease and exonuclease, none detected
• NICKase and DNase, none detected
• Optimal pH: 7.6 (activity range of 6-10)
• Optimal temperature: 60°C (activity range of 15-70°C)
• Shelf life: 3 years (-20°C)

Transportation and Storage

Storage temperature: -20°C.

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