Ribonuclease A Recombinant

Product Description:

Our Ribonuclease A Recombinant is a high-quality enzyme produced through recombinant technology, designed for the specific degradation of RNA molecules. This enzyme is widely used in molecular biology applications to remove RNA contaminants or to prepare RNA-free solutions.

Key Features:

• Produced through recombinant technology for high purity
• Efficient degradation of RNA molecules
• Suitable for DNA isolation and purification protocols
• Effective in eliminating RNA contamination from DNA samples
• Compatible with a broad range of buffer conditions
• Ideal for preparing DNA samples for downstream applications

Applications:

• DNA isolation and purification to remove contaminating RNA
• Preparation of DNA samples for PCR and other DNA-based assays
• Elimination of RNA from protein purification processes
• Quality control of DNA samples prior to sequencing or microarray analysis
• Educational and research settings for molecular biology experiments
• Clinical and diagnostic laboratories for DNA sample preparation

Our Ribonuclease A Recombinant is a reliable tool for researchers who need to ensure the absence of RNA in their DNA samples. Its high efficiency and compatibility with various buffers make it a versatile choice for a wide range of molecular biology workflows.

Description

Ribonuclease A (RNase A) is a genetically engineered endonuclease which is originally from bovine genome. The product is produced and purified from yeast cells without contamination of endotoxin.

RNase A is a very stable endoribonuclease and a single chain polypeptide containing four disulfide bridges. Solutions of RNase A have been reported to withstand temperatures up to 100°C. The highest activity is exhibited with single stranded RNA. Activators of RNase A include potassium and sodium salts.

A major application for RNase A is the removal of RNA from preparations of biological products, such as plasmid DNA or protein samples. It is also used in RNA sequence analysis and protection assays.

• CAS No.: 9001-99-4
• E.C.: 3.1.27.5
• Source: Pichia pastoris cells with cloned gene encoding genetically engineered Bovine pancreatic ribonuclease.
• Concentration: 10 mg/mL
• Activity: ≥ 350 U/μL
• Molecular weight: 14.3 kDa
• Form: Liquid: clear colorless buffered aqueous glycerol solution
• Grade: Molecular biology level
• Purity: ≥ 90%; (SDS-PAGE)
• Endotoxin: None detected (Tachypleus Amebocyte Lysate)
• Foreign activity: Endonuclease and exonuclease, none detected
• NICKase and DNase, none detected
• Optimal pH: 7.6 (activity range of 6-10)
• Optimal temperature: 60°C (activity range of 15-70°C)
• Shelf life: 1 year (-20°C)

Transportation and Storage

Storage temperature: -20°C.
Storage buffer: 10 mM Tris-HCl (pH 8.0), 50% glycerol.
Dilution buffer: 10 mM Tris-HCl (pH 8.0).

Procedure

RNase A is provided as a solution of 10 mg/mL.
RNase A can be used at a final concentration of 10-20 μg/mL.

Order