HotStar DNA Polymerase bulk pack for IVD
- Introduction
Introduction
Anti body modifed Hotstar DNA Polymerase, suitable for IVD use
Description
The Hot Start Taq DNA Polymerase is a recombinant Taq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme need to be activated in incubation at 95°C for 10 minute. The activated enzyme maintains the same functionality.
Taq DNA polymerase: Has the 5' to 3' DNA polymerase activity and 5' to 3' exonuclease activity without 3' - 5' exonuclease activity. The extending speed is 1 kb/30 sec. There is an "A" on 3' end. The PCR product can be cloned in TA vector.
Applications
- Hot-start PCR amplification
- Specific amplification of complex cDNA and genomic template
- Amplification from low copy number DNA template
- Real-Time PCR
- Multiple PCR
- Generation of PCR products for TA cloning, Gene microarray analysis and SNP test
Quality Control
- Functional absence of double and single-stranded endonuclease activity;
- Purity>99% test by SDS gel electrophoresis;
- Each lot of HotStar Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA;
- Retain full activity at room temperature for one week;
- No host DNA residue.
Concentration: 5 u/μl
Package: Bulk
Storage: Store at -20°C
Order
PC11 | Hotstar DNA Polymerase | Anti body modifed Hotstar DNA Polymerase, suitable for IVD use |
PC12 | E star Taq DNA Polymerase | Double Anti body modifed Hotstar DNA Polymerase, suitable for IVD use |