HiFi II M-MLV Reverse Transcriptase(RNase H-)

Product Description:

Our HiFi II M-MLV Reverse Transcriptase (RNase H-) is a highly efficient enzyme designed for reverse transcription applications that require minimal RNA degradation. This enzyme is a modified version of the Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, lacking RNase H activity, which prevents the degradation of the RNA template during cDNA synthesis.

Key Features:

• Modified M-MLV reverse transcriptase with no RNase H activity
• High fidelity and efficiency in cDNA synthesis
• Suitable for reverse transcription of full-length mRNA
• Reduced RNA degradation for better template preservation
• Compatible with a wide range of RT-PCR buffer conditions
• Ideal for generating high-quality cDNA for downstream applications

Applications:

• Reverse transcription PCR (RT-PCR)
• Synthesis of cDNA libraries for sequencing and cloning
• Gene expression analysis
• Detection and quantification of RNA viruses
• Amplification of rare or low-abundance RNA transcripts
• Educational and research settings for RNA studies

Our HiFi II M-MLV Reverse Transcriptase (RNase H-) is an essential tool for researchers conducting reverse transcription experiments. Its lack of RNase H activity ensures that RNA templates remain intact, leading to more accurate and reliable cDNA synthesis.

Description

MMLV Reverse Transcriptase, encoded by Moloney Murine Leukemia Virus (MMLV RT) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. MMLV RT will also extend primers hybridized to single-stranded DNA. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase. The difference between this to the general M-MLV RT is that the capacity to endure the heat is enhanced. It can remain the 100% activity at 50°C, it can also keep more than 80% activity even at 55°C.

Source

Recombination of E. coli containing Moloney murine leukemia virus reverse transcriptase gene from clone of Moloney murine.

Applications

The first-strand cDNA synthesis; RT-PCR

Features

• lack RNase H activity: Weak RNase H activity High cDNA yield, can get more full length cDNA.
• thermoStable: the optimum reaction temperature is 50°C, the highest is 60°C. Can overcome the template RNA secondary structure, and finish the reverse transcriptase experiment smoothly.
• wide temperature range: can reverse transcript from 37-60C, with more than 80% of the highest activity at 42°C-55°C customer can choose the reaction temperature freely.
• Strong amplification activity: Gene mutation enhanced the binding capacity of the enzyme and RNA. Increased the amplification speed, can obtain the quality cDNA, suitable for cDNA library construction.

Unit Definition

One unit of MMLV RT catalyzes the incorporation of 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using oligo(dT)12-18-primed poly(A)n as a template.

Concentration: 200U/μl

Package: Bulk

Component: M-MLV (200U/μl) 5xBuffer (with DTT)

Storage: -20°C

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