E star Taq DNA Polymerase

Product Description:

Our E star Taq DNA Polymerase is a cutting-edge enzyme developed for superior performance in PCR applications. This enzyme is engineered to offer enhanced speed, specificity, and sensitivity, making it a top choice for demanding molecular biology workflows.

Key Features:

• Engineered for rapid and specific amplification
• High specificity with reduced non-specific amplification
• Increased sensitivity for detecting low-abundance targets
• Suitable for a wide range of PCR buffer conditions
• Ideal for real-time PCR and quantitative analysis
• Compatible with automated liquid handling systems

Applications:

• Routine PCR and RT-PCR
• Real-time PCR and quantitative analysis
• Amplification of low-copy number templates
• Diagnostic assays and pathogen detection
• Forensic analysis and genetic testing
• Educational and research settings

Our E star Taq DNA Polymerase is a powerful tool for achieving accurate and reliable results in PCR and RT-PCR assays. Its advanced properties ensure that researchers can amplify DNA targets with precision and confidence, even in challenging scenarios.

Description

The Hot Start Taq DNA Polymerase is a recombinant Taq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme need to be activated in incubation at 95°C for 10 minute. The activated enzyme maintains the same functionality.

Taq DNA polymerase: Has the 5' to 3' DNA polymerase activity and 5' to 3' exonuclease activity without 3' - 5' exonuclease activity. The extending speed is 1 kb/30 sec. There is an "A" on 3' end. The PCR product can be cloned in TA vector.

Applications

• Hot-start PCR amplification
• Specific amplification of complex cDNA and genomic template
• Amplification from low copy number DNA template
• Real-Time PCR
• Multiple PCR
• Generation of PCR products for TA cloning, Gene microarray analysis and SNP test

Quality Control

• Functional absence of double and single-stranded endonuclease activity;
• Purity>99% test by SDS gel electrophoresis;
• Each lot of HotStar Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA;
• Retain full activity at room temperature for one week;
• No host DNA residue.

Concentration: 5 u/μl

Package: Bulk

Storage: Store at -20°C

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